How to Choose Chemical & Microbial Protective Gloves Under EN 374 Standards: A 2025 Guide

en 374 standard

Selecting gloves compliant with EN 374 standards is critical for safely handling hazardous chemicals or microorganisms in industrial, medical, and laboratory settings. This guide breaks down the latest EN ISO 374:2016 requirements and provides actionable steps to choose the right gloves.

Why EN 374 Standards Matter for Safety

EN 374 is the EU’s benchmark for evaluating gloves against chemical penetration, permeation, and degradation, ensuring reliable protection in high-risk environments. Key updates in the 2016 revision include:

  • Virus protection requirements (using ISO 16604 Method B)
  • Expanded testing to 18 chemicals (codes A-T, up from 12)
  • Revised classification and labeling rules (References: EN 374-1:2016) 

Key Updates & Selection Criteria

1. Glove Classification & Performance Levels

  • Type A: Resists ≥6 chemicals for ≥30 minutes (e.g., labs, chemical plants)
  • Type B: Resists ≥3 chemicals for ≥30 minutes (moderate-risk tasks)
  • Type C: Resists ≥1 chemical for ≥10 minutes (low-risk/short-term use)

2. Expanded Chemical Testing (Codes M-T)

Newly added chemicals include nitric acid (M), hydrogen peroxide (Q), and formaldehyde (T). Action Step: Verify glove labels for specific chemical codes. For example, healthcare workers handling formaldehyde must choose gloves with Code T certification.

3. Enhanced Microbial Protection

  • Bacteria/Fungi: Passes EN 374-2 penetration tests.
  • Viruses: Requires ISO 16604 Method B validation (using bacteriophage ΦX174).  

4. Material Degradation Testing (EN 374-4)

Assesses physical changes (swelling, brittleness) after chemical exposure. For instance, nitrile gloves may soften when exposed to oils. Pro Tip: Prioritize gloves with published degradation data.

5. Cuff Design Requirements

  • Gloves ≥40 cm long must undergo cuff permeability testing (80 mm from cuff edge).
  • Non-chemical-resistant cuffs must be clearly labeled. Use Case: Long-cuff gloves are ideal for chemical decontamination, while short cuffs suit quick tasks.  

5-Step Selection Process

  1. Identify Hazards: Determine chemical types (e.g., solvents, acids) or microbial risks.
  2. Match Protection Level: Use Type A/B/C based on exposure duration and toxicity.
  3. Verify Certifications: Look for EN ISO 374-1:2016 and EN 16523-1:2015 labels.
  4. Assess Usage Conditions: Factor in temperature, abrasion, and degradation data.
  5. Review Documentation: Confirm chemical codes, cuff specs, and virus test results.

Common Myths & Best Practices

❌ Myth 1: “Type C Gloves Are Reusable”

Truth: Type C offers minimal protection (≥10 minutes). Replace hourly if handling fast-penetrating chemicals.

❌ Myth 2: “All Nitrile Gloves Block Viruses”

Truth: Only nitrile gloves passing ISO 16604 qualify for viral protection.

✅ Pro Tips:

  • Test glove compatibility for chemical mixtures (synergistic effects may reduce performance).
  • Prioritize aged degradation data over initial test results for long-term reliability.  

Conclusion

EN 374 standards provide a science-backed framework for selecting gloves that balance safety and practicality. By aligning glove type with specific risks, verifying certifications, and monitoring degradation, professionals across industries can minimize exposure hazards.

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